Finsen 2004
Alle deler > ALEXANDRA
Finsen AV, Woldbaek PR, Li J, Wu J, Lyberg T, Tønnessen T, Christensen G.
INCREASED SYNDECAN EXPRESSION FOLLOWING MYOCARDIAL INFARCTION INDICATES A ROLE IN CARDIAC REMODELING.
Physiol Genomics 2004; 16: 301-8.
The purpose of this study was to identify
essential genes involved in myocardial growth and remodeling following
myocardial infarction (MI). Left ventricular noninfarcted tissues from six mice
subjected to MI under general anesthesia and from six sham-operated mice were
obtained 1 wk after primary surgery and analyzed by means of cDNA filter
arrays. Out of a total of 1,176 genes, 641 were consistently expressed,
twenty-three were upregulated and thirteen downregulated. Five genes were only
expressed following MI. Syndecan-3, a transmembranous heparan sulfate
proteoglycan, was found to be upregulated together with a transcriptional
activator of syndecans, Wilms tumor protein 1 (WT-1). Northern blotting
demonstrated a significant upregulation of syndecan-1, -2, -3, and -4, WT-1,
fibronectin, and basic fibroblast growth factor (FGF) receptor 1. Furthermore,
Western blot analysis showed statistically significant increases in protein
levels for syndecan-3 and -4. In conclusion, we have identified a subset of genes
with increased expression in noninfarcted left ventricular tissue following MI,
including syndecans 1-4, WT-1, fibronectin, collagen 6A, and FGF receptor 1.
Since the syndecans link the cytoskeleton to the extracellular matrix and
function as required coreceptors for FGF, we suggest a role for the syndecans
in cardiac remodeling following MI.